(vasep.com.vn) Scientists in Saigon Hi-tech Park have announced to produce successfully an enrofloxacin quick detecting kit.
Enrofloxacin is a kind of antibiotic that is banned for fish aquaculture in Vietnam because it is harmful to human.
The kit is based on the competitive enzyme immunoassay. The coupling antigens is pre-coated on the micro-well stripes. The enrofloxacin residue in the sample and coupling antigens pre-coated on the micro-well stripes compete for the anti-enrofloxacin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the content of enrofloxacin residue in it. This value is compared to the standard curve and the content of the corresponding enrofloxacin residue is subsequently obtained.
The extraction of enrofloxacin from fish can be finished in 30 minutes which is faster than other methods.
The kit has high sensitivity; its detection limit is 1ppb and it can analyze 50-80 samples in one time. Besides, the kit provides a unique extraction method, which is accurate, rapid, cost effective and easy to use.