Researchers struggling to develop EMS test

News 14:44 13/09/2014 496
University of Arizona researchers working hard to define bacteria. Researchers at the University of Arizona are having problems creating a reliable diagnostic test for early mortality syndrome (EMS) due to the similarity of the EMS-causing bacteria to many others in the marine environment.

 "We have been working very hard on getting a reliable diagnostic test for EMS/AHPNS to market, but unfortunately we are working with Vibrio parahaemolyticus, which is very common in the marine environment, so first we have to figure out what genetic element is different in this Vibrio," wrote Linda Nunan, an assistant staff scientist at Donald Lightner's shrimp pathology laboratory at The University of Arizona, on The Shrimp List -- a mailing list for the shrimp farming industry.

Nunan said she has an experimental PCR test that detects the strain in both Asia and Mexico broth cultures, but she is still in the process of validating it, and did not feel comfortable releasing a protocol that may cross react with non-EMS causing Vibrios. "Believe me, I wish this disease had been caused by a somewhat more unique species of bacteria, which would have made developing a diagnostic test much easier," she wrote.

"My primary concern is to develop a validated, reliable test so we can screen broodstock, PLs and imports and exports for the disease.  This will help stop the spread of the disease.  Shrimp farmers are in a much better position than we are to determine which pond treatments work best."

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